GFP Tagged HEK293 Cells

Size Price Quantity
1.0 Frozen Vial $750.00
Name of Products GFP Tagged Human Embryonic Kidney Cells (GFP-HEK293)
Catalogue NumbercAP-0200GFP
Product FormatFrozen Vial
Cell Number>5x10[5] cells/vial)
HEK293 cells (cAP-0200) is an epithelial-like cell line derived from human fetal kidney tissue. GFP-HEK293 cells are selected from HEK293 cells infected by GFP expressing lentiviruses (under the control of CMV promoter) by puromycin (2-4 ug/ml). The cells are shipped in frozen vials (the cells are provided @ earlier passage). Universal Growth Medium (UGM, contains 10% FBS, cAP-01B) is recommended for cell culture and these cells have a minimum average population doubling levels > 40 when cultured following the detailed protocol described below).
GFP-HEK293 cells are for research use only.
Frozen Vials.
When you receive the cells in a frozen vial, you can transfer the vial of cells into a -80ºC freezer for short period storage or a liquid nitrogen tank for long term storage. Thaw the cells in a 37°C water bath, and then transfer the cells in a T25 flask pre-coated with Quick coating solution (cAP-01) as described in detail in Subculture Protocol.
A)Pre-coating of T25 flasks
B)Rinse the cells in T25 flask with 5ml HBSS (Room Temperature, RT) twice.
C)Add 2ml of Trypsin/EDTA (RT) (cAP-23) into one T25 flask (make sure the whole surface of the T25 flask is covered with Trypsin/EDTA), and gently dispose the excessive Trypsin/EDTA solution within 20 seconds with aspiration.
D)Leave the T25 flask with the cells at RT for 1 minute (the cells usually will detach from the surface within 1-2 minutes). You can monitor the cells under microscope and when most of cells become rounded up, hit the flask against the bench surface, and the cells will move on the surface of the flask when monitoring under a microscope.
E)Add 5ml Trypsin Neutralization Buffer and spin the cells down with 800g for 5 minutes.
F)Re-suspend the cell pellet with 15ml of UGM full medium and the cell suspension is transferred directly into 3 pre-coated T25 flasks (5ml each, and the cells are sub-cultured at 1
G)Change medium every 2-3 days and cells usually become confluent within 7 days (when split at a 1
Quick Coating Solution cAP-01 240ml
Universal Growth Medium cAP-01B 500ml
Universal Basal Medium cAP-01C 500ml
HBSS w/o Ca2+ , Mg2+ cAP-11 100ml
Cell Freezing Solution (FBS) cAP-22 50ml
Cell Freezing Solution (Non-FBS) cAP-22B 50ml
Trypsin/EDTA Solution cAP-23 100ml
Trypsin Neutralization Solution cAP-28 100ml
ITS (100x) cAP-26 10ml
L-Glutamine-MAXIMUM (100x) cAP-27 100ml
Human Plasma Fibronectin Solution cAP-42 1mg/ml
Handling the human tissue-derived products is potentially bio-hazardous. Although each cell strain is tested negative for HIV, HBV and HCV DNA, diagnostic tests are not necessarily 100% accurate; therefore, proper precautions must be taken to avoid inadvertent exposure. Always wear gloves and safety glasses when working these materials. Never mouth pipette. We recommend following the universal procedures for handling products of human origin as the minimum precaution against contamination.
SPECIAL NOTES: 1. We strongly advise our customers using our own medium and related accessory products; 2. The quality of cells, in term of their growth features stated in our datasheets, will not be waranteed if NON-ANGIO-PROTEOMIE's Cell Growth Medium is used; 3.Due to the sensitive nature of primary cells and cell lines, all quality related issues about the cells products must be reported back to us within ONE month period after receiving the products, no quality warranty (i.e. replacement of cells) will be provided after the ONE-Month period.