HEK293 Cells

Size Price Quantity
1.0 Frozen Vial $400.00
Name of Products Human Embryonic Kidney Cells (HEk293)
Catalogue NumbercAP-0200
Product FormatFrozen Vial
Cell Number>5x10[5] cells/vial)
HEK293 cells (cAP-0200) is an epithelial like cell line derived from human fetal kidney tissue. The cells express the transforming gene of adenovirus 5. Adenovirus 5 DNA from both the right and left ends of the viral genome are present. The line is excellent for titrating human adenoviruses. The cells are shipped in frozen vials (the cells are provided @ earlier passage). Universal Growth Medium (UGM, contains 10% FBS, cAP-01B) is recommended for cell culture and these cells have a minimum average population doubling levels > 40 when cultured following the detailed protocol described below).
HEK293 cells are for research use only.
Frozen Vials.
When you receive the cells in a frozen vial, you can transfer the vial of cells into a -80ºC freezer for short period storage or a liquid nitrogen tank for long term storage. Thaw the cells in a 37°C water bath, and then transfer the cells in a T25 flask pre-coated with Quick coating solution (cAP-01) as described in detail in Subculture Protocol.
A)Pre-coating of T25 flasks
B)Rinse the cells in T25 flask with 5ml HBSS (Room Temperature, RT) twice.
C)Add 2ml of Trypsin/EDTA (RT) (cAP-23) into one T25 flask (make sure the whole surface of the T25 flask is covered with Trypsin/EDTA), and gently dispose the excessive Trypsin/EDTA solution within 20 seconds with aspiration.
D)Leave the T25 flask with the cells at RT for 1 minute (the cells usually will detach from the surface within 1-2 minutes). You can monitor the cells under microscope and when most of cells become rounded up, hit the flask against the bench surface, and the cells will move on the surface of the flask when monitoring under a microscope.
E)Add 5ml Trypsin Neutralization Buffer and spin the cells down with 800g for 5 minutes.
F)Re-suspend the cell pellet with 15ml of UGM full medium and the cell suspension is transferred directly into 3 pre-coated T25 flasks (5ml each, and the cells are sub-cultured at 1
G)Change medium every 2-3 days and cells usually become confluent within 7 days (when split at a 1
Quick Coating Solution cAP-01 240ml
Universal Growth Medium cAP-01B 500ml
Universal Basal Medium cAP-01C 500ml
HBSS w/o Ca2+ , Mg2+ cAP-11 100ml
Cell Freezing Solution (FBS) cAP-22 50ml
Cell Freezing Solution (Non-FBS) cAP-22B 50ml
Trypsin/EDTA Solution cAP-23 100ml
Trypsin Neutralization Solution cAP-28 100ml
ITS (100x) cAP-26 10ml
L-Glutamine-MAXIMUM (100x) cAP-27 100ml
Human Plasma Fibronectin Solution cAP-42 1mg/ml
Handling human tissue derived products is potentially bio-hazardous. Although each cell strain is tested negative for HIV, HBV and HCV DNA, diagnostic tests are not necessarily 100% accurate; therefore, proper precautions must be taken to avoid inadvertent exposure. Always wear gloves and safety glasses when working these materials. Never mouth pipette. We recommend following the universal procedures for handling products of human origin as the minimum precaution against contamination.