| Catalog Number | hAP-1074 |
Rat Monoclonal anti-human PON1 |
| Size | 100ug |
| Immunogen | Recombinant human PON1 |
| Clone | MM1074-32L1 |
| Antibody Isotype | Rat IgG2 |
| Preparation | This antibody was produced from a hybridoma (rat myeloma fused with spleen cells) from a rat immunized with recombinant human PON1 (Paraoxonase-1) PON1 is also called arylesterase1 and serum paraoxonase and is a 43kDa glycoprotein that is expressed in liver and is secreted into the bloodstream where it associates with high-density lipoproteins (HDL). |
| Formulation and Storage | The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from the date of receipt when stored at -70C |
| Specificity | This antibody recognizes human PON1 in direct ELISAs and WBs. It cross reacts with mouse PON1 in WBs. |
| Reconstitution | Reconstitute the antibody with 500ul sterile PBS and the final concentration is 200ug/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles. |
| Applications | |
| A. | WB-(1 to 1000-2000) |
| B. | |
| C | |
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