Mouse Monoclonal anti-human HIF-1alpha

Item#:

hAP-1016

Size	Price	Quantity
50.0 ug	$420.00

Catalog Number	hAP-1016
Mouse Monoclonal anti-human HIF-1alpha
Size	100ug
Immunogen	Recombinant human HIF-1alpha
Clone	MM1016-10T1
Antibody Isotype	Mouse IgG1
Preparation	This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells) from a mouse immunized with recombinant human HIF-1alpha (Hypoxia-Inducible transcription Factor 1alpha). HIF-1alphalpha is the regulated member of the transcription factor heterodimer HIF-1.
Formulation and Storage	The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from the date of receipt when stored at -70C
Specificity	This antibody recognizes human HIF-1alpha in direct ELISAs and WBs. It cross reacts with mouse and rat HIF-1alpha in WBs.
Reconstitution	Reconstitute the antibody with 500ul sterile PBS and the final concentration is 200ug/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Applications
A.	WB-(1 to 1000-2000)
B.	IHC-P-(1 to 100-200)
C.	FC-Yes
D.