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Sheep Polyclonal anti-human IL-10

Size Price Quantity
50.0 ug $245.00
Catalog Number hAP-5253
Sheep Polyclonal anti-human IL-10
Size 50ug
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-10.
Clone Polyclonal AB
Antibody Isotype IgG
Preparation This antibody was produced from a sheep immunized with purified human IL-10 protein.
Formulation and Storage The IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from the date of receipt when stored at -70C
Specificity Detects human IL-10 in direct ELISAs and Western blots. In direct ELISAs, approximately 75% crossreactivity with recombinant Epstein-Barr virus IL-10 is observed, approximately 15% cross-reactivity with recombinant mouse IL-10, recombinant feline IL-10, recombinant canine IL-10, recombinant porcine IL-10, and recombinant equine IL-10 is observed and less than 5% cross-reactivity with recombinant rat IL-10, recombinant cotton rat IL-10, and recombinant guinea pig IL-10 is observed.
Reconstitution Reconstitute the antibody with 100 ul sterile PBS and the final concentration is 500ug/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
A.WB-(1 to 1000-2000)
B.IHC(Paraffin/Frozen)-(1 to 50-100)