| |
| Name of Products | RFP-SV40-Rat Brain Microglia Cells |
| Catalog Number | cAP-r0006RFP |
| Product Format | Frozen Vial |
| Cell Number | > 5x10[5] cells/vial |
| Freshly isolated Rat (Sprague Dawley) Brain microglia cells (RBMgs) were isolated from healthy rat brain tissue. After purification, RBMgs were infected with lentiviruses expressing SV40T and RFP, and puromycin- and Zeocin-resistant cells were selected and cryopreserved. SV40-RBMgs can be expanded in Rat Microglia Growth Medium (cAP-r006M). |
| |
| 1. | Tomato Lectin Positive |
| 2. | IBA1 Positive |
| 3. | OX42 Positive |
| 4. | RBMgs are negative for mycoplasma. |
| The cells are offered for Research Use Only. |
| Frozen Vials in a Dry Ice Package. |
| When you receive the cells in a frozen vial, you can transfer the vial of cells into a -80C freezer for short-period storage or a liquid nitrogen tank for long-term storage. Thaw the cells in a 37C water bath, and then transfer the cells into a T75 flask as described in the following details. |
| |
| A) | Warm Rat Microglia Growth Medium (RMGM, cAP-r0006M) before thawing the cells. |
| B) | Place the Frozen vial of the cells in a 37C water bath, and remove the vial from the water bath immediately. |
| C) | Dispense the contents of the vial into 10ml of full medium and spin down the cells at 1000RPM for 8 mins |
| D) | Resuspended the cells with 10ml of the full medium and transfer the cells into 1 T75 flask. |
| E) | Return the culture vessels to the incubator (For best result, do not disturb the culture for at least 16 hours after the culture has been initiated. |
| G) | Change the medium every two to three days thereafter. |
| F) | To passage the cells, rinse the cells in a T75 flask with 10ml HBSS (RT) twice; then add 2ml Trypsin/EDTA (RT) (cAP-23) into one T75 flask; gently dispose the excessive Trypsin/EDTA solution within 20 seconds by aspiration. |
| I) | Leave the T75 flask with the cells at RT or 37C for 1 min (most cells usually will detach from the surface within 1-2 mins; or monitor the cells under a microscope until most of cells become rounded up, and then gently tap the flask against the bench surface, and the cells will move on the surface of the flask when monitoring under microscope. |
| J) | Add 10ml Trypsin Neutralization Buffer and spin down the cells with 1000rpm centrifugation for 8 mins. |
| K) | Re-suspend the cell pellet with 30ml cAP-r0006M and transfer 10ml each into 3 T75 flasks. |
| L) | Change medium every 2 or 3days untl the cells reach to 95% confluence. |
| |
| Rat Microglia Growth Medium | cAP-r0006M |
| HBSS w/o Ca2+, Mg2+ | cAP-11 |
| Cell Freezing Solution (FBS) | cAP-22 |
| Cell Freezing Solution (Non-FBS) | cAP-22B |
| Trypsin/EDTA Solution | cAP-23 |
| Trypsin Neutralization Solution | cAP-28 |
| ITS (100x) | cAP-26 |
| L-Glutamine-MAXIMUM (100x) | cAP-27 |
| Human Plasma Fibronectin Solution | cAP-42 |
| Handling human/animal tissue-derived products is potentially bio-hazardous, despite tested negative for HIV, HBV and HCV DNA; nonetheless, proper precautions must be taken to avoid inadvertent exposure. |
For customers using our primary cell products, we strongly advise you using our own medium, although similar cell growth medium can be purchased and used for our primary cell products. |
The quality of cells, in term of their growth features stated in our datasheets, will not be guaranteed if NON-ANGIO-PROTEOMIE's Cell Growth Medium is used. |
Due to the sensitive nature of primary cells and cell lines, all quality related issues about the cells products must be reported back to us within ONE month period after receiving the products, no quality warranty (i.e. replacement of cells) will be provided after the ONE-Month period. Thank you for your understanding.
|
