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Rat Monoclonal anti-Mouse TNF-a Antibody


Item#:
xAP-0832
Size Price Quantity
100.0 ug $320.00
Catalog Number xAP-0832
ID Rat Monoclonal anti-Mouse TNF-a Antibody
Antigen Description TNF-a is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-a. Monomeric mouse TNF-a is a 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD. TNF-a forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-a has also been described. TNF-a binding to surface receptors elicits a wide array of biologic activities including
Size 100ug
Immunogen E. coli-expressed, recombinant mouse TNF-a
Clone #MP6-XT22
Antibody Isotype Rat IgG1, ¿
Species Reactivity Rat
Preparation The antibody was purified by affinity chromatography.
Formulation The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS).
Application ELISA ICFC CyTOF® ICC - IHC-F WB
Storage & Handling Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 0.5mg/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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