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Rat Monoclonal anti-Mouse Ly-6A/E (Sca-1) Antibody


Item#:
xAP-0785
Size Price Quantity
100.0 ug $320.00
Catalog Number xAP-0785
ID Rat Monoclonal anti-Mouse Ly-6A/E (Sca-1) Antibody
Antigen Description Ly-6A/E is an 18 kD member of the Ly-6 multigene family also known as Sca-1. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses. The E13-161.7 antibody has been reported to be useful for immunohistochemical staining of frozen sections, immunoprecipitation, flow cytometry, and complement-mediated cytotoxicity.
Size 100ug
Immunogen mouse pre-T cells
Clone #E13-161.7
Antibody Isotype Rat IgG2a, ¿
Species Reactivity Rat
Preparation The antibody was purified by affinity chromatography.
Formulation The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS).
Application FC IHC-F IHC-P IP
Storage & Handling Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 0.5mg/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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