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Rat Monoclonal anti-Mouse IL-10 Antibody

Size Price Quantity
100.0 ug $320.00
Catalog Number xAP-0754
ID Rat Monoclonal anti-Mouse IL-10 Antibody
Antigen Description IL-10 was first described as a cytokine that is produced by T helper 2 (Th2) cell clones. It inhibits interferon gamma (IFN-¿) synthesis in Th1 cell, and therefore it was initially called cytokine synthesis inhibiting factor (CSIF). Macrophages are the main source of IL-10, and its secretion can be stimulated by endotoxin (via Toll-like receptor 4, NF-¿B dependent), tumor necrosis factor TNF-a (via TNF receptor p55, NF-¿B-dependent), catecholamines, and IL-1. IL-10 controls inflammatory processes by suppressing the expression of proinflammatory cytokines, chemokines, adhesion molecules as well as antigen-presenting and costimulatory molecules in monocytes/macrophages, neutrophils, and T cells. IL-10 inhibits the production of proinflammatory mediators by monocytes and macrophages such as endotoxin- and IFN¿-induced release of IL-1a, IL-6, IL-8, G-CSF, GM-CSF, and TNF-a. In addition, it enhances the production of anti-inflammatory mediators such as IL-1RA and soluble TNF-a receptors. IL-10 inhibits the capacity of monocytes and macrophages to present antigen to T cells. This is realized by downregulation of constitutive and IFN¿-induced cell surface levels of MHC class II, of costimulatory molecules such as CD86, and of some adhesion molecules such as CD58.
Size 100ug
Immunogen Recombinant mouse IL-10
Clone #MK10A6
Antibody Isotype Rat IgG1
Host Species Rat
Preparation The antibody was purified by affinity chromatography.
Formulation The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS).
Application ELISA Capture
Storage & Handling Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 0.5mg/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.