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Mouse Monoclonal anti-Human/Mouse/Rat PCNA Antibody


Item#:
xAP-0482
Size Price Quantity
100.0 ug $400.00
Catalog Number xAP-0482
ID Mouse Monoclonal anti-Human/Mouse/Rat PCNA Antibody
Antigen Description The PC10 monoclonal antibody reacts with proliferating cell nuclear antigen also known as PCNA or the DNA polymerase d auxiliary protein. PCNA is a 36 kD trimeric ring that acts as a DNA-polymerase sliding clamp expressed in the nucleus of all proliferating cells. A prime function of PCNA appears to be increasing DNA polymerase d processibility during elongation of the leading strand. PCNA is a useful marker for DNA synthesis and is highly conserved among most species, thus highlighting the very broad reactivity of this antibody.
Size 100ug
Immunogen Recombinant rat PCNA
Clone #PC10
Antibody Isotype Mouse IgG2a
Host Species Mouse
Preparation The antibody was purified by affinity chromatography.
Formulation The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS).
Application ICFC ICC IHC-P - IP WB IHC
Storage & Handling Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 0.5mg/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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