Mouse Monoclonal anti-Human/Mouse ZAP-70 Antibody

Item#:

xAP-0480

Size	Price	Quantity
100.0 ug	$400.00

Catalog Number	xAP-0480
ID	Mouse Monoclonal anti-Human/Mouse ZAP-70 Antibody
Antigen Description	ZAP-70 is a 70 kD protein tyrosine kinase associated with the zeta chain of the T cell receptor. It is expressed in T cells and NK cells and has been shown to be involved in T cell signaling. Defects in ZAP-70 have been linked to selective T cell defects. The ZAP-70 kinase undergoes multiple phosphorylation events after T cell receptor engagement and interacts with a number of proteins involved in signal transduction. Recently, ZAP-70 has been identified as an important prognostic marker in B-cell chronic lymphocytic leukemia (B-CLL). The 1E7.2 monoclonal antibody recognizes human and mouse ZAP-70 and has been shown to be useful for flow cytometry, Western blotting, and immunoprecipitation.
Size	100ug
Immunogen	GST-fusion protein corresponding to residues 282-307 of human ZAP-70
Clone	#1E7.2
Antibody Isotype	Mouse IgG1
Host Species	Mouse
Preparation	The antibody was purified by affinity chromatography.
Formulation	The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS).
Application	ICFC WB IP
Storage & Handling	Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 0.5mg/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.