Mouse Monoclonal anti-Human Uncarboxylated Osteocalcin Antibody

Item#:

xAP-0470

Size	Price	Quantity
100.0 ug	$320.00

Catalog Number	xAP-0470
ID	Mouse Monoclonal anti-Human Uncarboxylated Osteocalcin Antibody
Antigen Description	Osteocalcin is the most abundant non-collagenous protein found in the bone. While fully-carboxylated osteocalcin has a high affinity for the extracellular matrix of the bone; decreases in pH, caused by the process of bone resorption, result in decarboxylation of this protein. This generates both partially decarboxylated (undercarboxylated) and fully decarboxylated (uncarboxylated) osteocalcin molecules which are released into the circulation due to decreased affinity for the extracellular matrix. Fully carboxylated osteocalcin requires high calcium concentrations for proper protein folding; however, under/uncarboxylated osteocalcin has no calcium requirement to maintain its structure within the blood.
Size	100ug
Immunogen	Recombinant Human Decarboxylated (3xGLU) Osteocalcin
Clone	#8H4
Antibody Isotype	Mouse IgG1
Host Species	Mouse
Preparation	The antibody was purified by affinity chromatography.
Formulation	The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS).
Application	ELISA Capture
Storage & Handling	Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 0.5mg/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.