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| Name of Products | Insulin Secretion Fibroblast-Like Transformed Human Pancreatic Islet Cells (IS-FLTHPICs) |
| Catalog No | cAP-0137 |
| Product Format | Frozen Vial |
| Cell Number | > 5x10[5] cells/vial |
| Insulin Secretion Fibroblast-Like Transformed Human Pancreatic Islet Cells (IS-FLTHPICs) was derived from a Human Pancreatic Islet Cells transformed with a proprietary method. The IS-FLTHPICs can be expanded in the Universal Growth Medium (cAP-01B) and Insulin is detected in the culture supernant (20-30pmol). Cells are supplied in frozen vials with more than 5 x 10[5] cell/vial. Universal Full Growth Medium (cAP-01B) is recommended to culture the cells. Cells are guaranteed to be passaged for 5 times at 1 to 3 split ratio |
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| 1. | IS-FLTHPICs are tested negative for HIV-1, HBV, HCV, and mycoplasma. |
| The cells are offered for Research Use Only. |
| Frozen Vials in a Dry Ice Package. |
| When you receive the dry ice package with cells in frozen vials, transfer the frozen vials of cells into a -80C freezer for short period storage or a liquid nitrogen tank for long-term storage. |
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| A) | Pre-coating of T75 flasks- Add 3-5ml each Quick Coating Solution (cAP-01) into a T75 flask to cover the whole surface of the flask, 5 mins later, aspirating off the excessive coating solution and the flask is ready to be used (although solution containing other extracellular matrices, i.e. gelatin, collagen, and fibronectin, can be used, make sure to optimize the conditions in advance). |
| B) | Thaw the frozen cell vial in a 37C water bath first, and then transfer the cells into the pre-coated T25 flask with 15ml of Full medium, cells usually become confluent within 5-7 days. |
| C) | To passage the cells, rinse the cells in the T75 flask with 15ml HBSS (RT) twice; then add 2ml Trypsin/EDTA (RT) (cAP-23) into one T75 flask; gently disposing excessive Trypsin/EDTA solution within 20 seconds by aspiration. |
| D) | Leave the flask with the cells at RT or 37C for 1 min (most cells usually will detach from the surface within 1-2 mins, and monitor the cells under a microscope until most of the cells become rounded up, and then gently tap the flask against the bench surface, and the cells will move on the surface of the flask when monitoring under a microscope. |
| E) | Add 5ml Trypsin Neutralization Buffer and spin down the cells with 800g centrifugation for 5 mins. |
| G) | Re-suspend the cell pellet with 10 or 15ml Full medium and transfer 5 ml each into 2 or 3 pre-coated T25 flasks (for 1/2 to 1/3 subculture ratio). |
| H) | Change medium every 2 or 3days and the cells usually become confluent within 7 days (when split at a 1/3 ratio). |
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| Quick Coating Solution | cAP-01 |
| Universal Full Growth Medium | cAP-01B |
| ITS Solution (100x) | cAP-26 |
| (HBSS w/o Ca2+, Mg2+ | cAP-11 |
| Cell Freezing Solution (FBS) | cAP-22 |
| Cell Freezing Solution (Non-FBS) | cAP-22B |
| Trypsin/EDTA Solution | cAP-23 |
| Trypsin Neutralization Solution | cAP-28 |
| ITS (100x) | cAP-26 |
| L-Glutamine-MAXIMUM (100x) | cAP-27 |
| Human Plasma Fibronectin Solution | cAP-42 |
| Handling human tissue-derived products is potentially bio-hazardous, despite tested negative for HIV, HBV and HCV DNA; nonetheless, proper precautions must be taken to avoid inadvertent exposure. |
For customers using our primary cell products, we strongly advise you using our own medium, although similar cell growth medium can be purchased and used for our primary cell products. |
The quality of cells, in term of their growth features stated in our datasheets, will not be guaranteed if NON-ANGIO-PROTEOMIE's Cell Growth Medium is used. |
Due to the sensitive nature of primary cells and cell lines, all quality related issues about the cells products must be reported back to us within ONE month period after receiving the products, no quality warranty (i.e. replacement of cells) will be provided after the ONE-Month period. Thank you for your understanding.
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