Home Page > PRIMARY CELL > GFP or RFP Labeled Cells >  Insulin Secretion Fibroblast-Like Transformed Human Pancreatic Islet Cells

Insulin Secretion Fibroblast-Like Transformed Human Pancreatic Islet Cells

Size Price Quantity
1.0 Frozen Vial $1,500.00
Name of Products Insulin Secretion Fibroblast-Like Transformed Human Pancreatic Islet Cells (IS-FLTHPICs)
Catalog NocAP-0137
Product FormatFrozen Vial
Cell Number> 5x10[5] cells/vial
Insulin Secretion Fibroblast-Like Transformed Human Pancreatic Islet Cells (IS-FLTHPICs) was derived from a Human Pancreatic Islet Cells transformed with a proprietary method. The IS-FLTHPICs can be expanded in the Universal Growth Medium (cAP-01B) and Insulin is detected in the culture supernant (20-30pmol). Cells are supplied in frozen vials with more than 5 x 10[5] cell/vial. Universal Full Growth Medium (cAP-01B) is recommended to culture the cells. Cells are guaranteed to be passaged for 5 times at 1 to 3 split ratio
1. IS-FLTHPICs are tested negative for HIV-1, HBV, HCV, and mycoplasma.
The cells are offered for Research Use Only.
Frozen Vials in a Dry Ice Package.
When you receive the dry ice package with cells in frozen vials, transfer the frozen vials of cells into a -80C freezer for short period storage or a liquid nitrogen tank for long-term storage.
A)Pre-coating of T75 flasks- Add 3-5ml each Quick Coating Solution (cAP-01) into a T75 flask to cover the whole surface of the flask, 5 mins later, aspirating off the excessive coating solution and the flask is ready to be used (although solution containing other extracellular matrices, i.e. gelatin, collagen, and fibronectin, can be used, make sure to optimize the conditions in advance).
B) Thaw the frozen cell vial in a 37C water bath first, and then transfer the cells into the pre-coated T25 flask with 15ml of Full medium, cells usually become confluent within 5-7 days.
C)To passage the cells, rinse the cells in the T75 flask with 15ml HBSS (RT) twice; then add 2ml Trypsin/EDTA (RT) (cAP-23) into one T75 flask; gently disposing excessive Trypsin/EDTA solution within 20 seconds by aspiration.
D) Leave the flask with the cells at RT or 37C for 1 min (most cells usually will detach from the surface within 1-2 mins, and monitor the cells under a microscope until most of the cells become rounded up, and then gently tap the flask against the bench surface, and the cells will move on the surface of the flask when monitoring under a microscope.
E) Add 5ml Trypsin Neutralization Buffer and spin down the cells with 800g centrifugation for 5 mins.
G) Re-suspend the cell pellet with 10 or 15ml Full medium and transfer 5 ml each into 2 or 3 pre-coated T25 flasks (for 1/2 to 1/3 subculture ratio).
H) Change medium every 2 or 3days and the cells usually become confluent within 7 days (when split at a 1/3 ratio).
Quick Coating SolutioncAP-01
Universal Full Growth MediumcAP-01B
ITS Solution (100x)cAP-26
(HBSS w/o Ca2+, Mg2+cAP-11
Cell Freezing Solution (FBS)cAP-22
Cell Freezing Solution (Non-FBS)cAP-22B
Trypsin/EDTA SolutioncAP-23
Trypsin Neutralization SolutioncAP-28
ITS (100x)cAP-26
L-Glutamine-MAXIMUM (100x) cAP-27
Human Plasma Fibronectin SolutioncAP-42
Handling human tissue-derived products is potentially bio-hazardous, despite tested negative for HIV, HBV and HCV DNA; nonetheless, proper precautions must be taken to avoid inadvertent exposure.