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Fibroblast Growth Factor-2, Human Recombinant


Item#:
rAP-6003
Size Price Quantity
1.0 mg $1,000.00
Product Name Fibroblast Growth Factor-2, Human Recombinant
Synonyms Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.
Description Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 154 amino acids and fused with His Tag and Thioredoxin at N-Terminal, having a combined molecular mass of 35kDa. The FGF-2 is purified by proprietary chromatographic techniques.
Uniprot Accesion Number P09038
Amino Acid SequenceAAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS.
Source Escherichia Coli.
Physical Appearance and Stability Lyophilized Fibroblast Growth Factor-2, although stable at room temperature for 3 weeks, should be stored desiccated below -20C. Upon reconstitution, FGF-b should be stored at 4C for less than 30 days, and for future use below -20C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Formulation and Purity The protein was lyophilized from a 0.2um filtered concentrated protein solution in PBS, pH 7.4. Greater than 98.0% as determined by SDS-PAGE.
Application
a.Cell culture medium (including, endothelial growth medium, iPSC serum-free medium et al
b. in vitro cell stimulation.
Solubility It is recommended to reconstitute the lyophilized Fibroblast Growth Factor Basic in 0.5ml sterile PBS, which can then be further diluted to other aqueous solutions.
Biological Activity The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5 ng/ml.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticide products, food additives or household chemicals.

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