| Product Name | Fibroblast Growth Factor-2, Human Recombinant |
| Synonyms | Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b. |
| Description | Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 154 amino acids and fused with His Tag and Thioredoxin at N-Terminal, having a combined molecular mass of 35kDa. The FGF-2 is purified by proprietary chromatographic techniques. |
| Uniprot Accesion Number | P09038 |
| Amino Acid Sequence | AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS. |
| Source | Escherichia Coli. |
| Physical Appearance and Stability | Lyophilized Fibroblast Growth Factor-2, although stable at room temperature for 3 weeks, should be stored desiccated below -20C. Upon reconstitution, FGF-b should be stored at 4C for less than 30 days, and for future use below -20C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Formulation and Purity | The protein was lyophilized from a 0.2um filtered concentrated protein solution in PBS, pH 7.4. Greater than 98.0% as determined by SDS-PAGE. |
| Application | |
| a. | Cell culture medium (including, endothelial growth medium, iPSC serum-free medium et al |
| b. | in vitro cell stimulation. |
| Solubility | It is recommended to reconstitute the lyophilized Fibroblast Growth Factor Basic in 0.5ml sterile PBS, which can then be further diluted to other aqueous solutions. |
| Biological Activity | The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5 ng/ml. |
| Shipping Format and Condition | Lyophilized powder at room temperature. |
| Usage Statement | Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticide products, food additives or household chemicals. |
