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Stable Fibroblast Growth Factor-Basic (bFGF) Human Recombinant


Item#:
rAP-2203A
Size Price Quantity
500.0 ug $650.00
1000.0 ug $1,250.00
Product Name Stable Fibroblast Growth Factor-Basic (bFGF) Human Recombinant
Synonyms Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.
Description Stable Fibroblast Growth Factor-basic (bFGF) Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids and fused with 6 x His-Tag and Thioredoxin (TRX) and having a molecular mass of 35.3kDa.The stable FGF-b is purified by proprietary chromatographic techniques.
Uniprot Accession Number P09038
Amino Acid Sequence 134AA (M154-S288)
Source Escherichia Coli.
Physical Appearance and Stability Lyophilized bFGF, although, stable at room temperature, should be stored desiccated below -20C. Upon reconstitution, bFGF could be stored at 4C for up to 4 weeks, and for future use, below -20C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Formulation and Purity The protein was lyophilized from a concentrated (7mg/ml) solution in PBS, pH 7.4 and, 300mM NaCl. Greater than 98.0% as determined by SDS-PAGE.
Application
Solubility It is recommended to reconstitute the lyophilized Fibroblast Growth Factor Basic in sterile PBS, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5 ng/ml, corresponding to a specific activity of 2,000,000 Units/mg. It has also been tested in iPSC medium stable bFGF is of > 120hrs half-life.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticide products, food additives, or household chemicals.

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