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| Name of Products | Human Colon Cancer Organoid |
| Catalogue Number | cAP-0205 |
| Product Format | Frozen Vial |
| Cell Number | > 3 x 10[6] cells/vial |
| Human Colon cancer tissue was procured from an approved source. Cells were isolated by collagenase digestion and cells were cultures with Matrigel (Corning) in 50/50 DMEM/F12 supplemented with 5%FBS, ROCK inhibitor (to inhibit the growth of fibroblasts), and bFGF. At this stage, cells grow as small organoids. The cells were passaged for a couple of times and cryopreserved. Organoids are cryopreserved in a commercial freezing medium with about 3 x 10[6] cancer cells as organoids per vial. |
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| 1. | Colon Cancer Organoid are negative for HIV-1, HBV, HCV, and mycoplasma |
| Cells are offered for Research Use Only. |
| Frozen Vials in a Dry Ice Package. |
| When you receive the dry ice package with cells in frozen vials, transfer the frozen vials of cells into a -80C freezer for short period storage or a liquid nitrogen tank for long- term storage. |
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| A) |
Frozen organoids are thawed at 37C with gentle shaking. |
| B) | The contents of the vial are
transferred to 5ml of tissue culture medium dropwise and spun at 500g and the viability after thawing is usually 85 to 90%. |
| C) | The organoids are plated in Ultra Low Binding tissue culture vessels (Corning). The cells are plated in 50/50 DMEM supplemented with 5% FBS, 10 ng/ml of basis FGF and antibiotics (25 ug/ml of gentamicin and 2.5 ug/ml of Amphotericin B). |
| D) | The organoids can either be used immediately for experiments or maintained for several weeks in this medium with periodic medium change (The organoids grow very slowly). |
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| Quick Coating Solution | cAP-01 |
| Astrocyte Growth Medium | cAP-29 |
| Astrocyte Basal Medium | cAP-29B |
| HBSS w/o Ca2+, Mg2+ | cAP-11 |
| Cell Freezing Solution (FBS) | cAP-22 |
| Cell Freezing Solution (Non-FBS) | cAP-22B |
| Trypsin/EDTA Solution | cAP-23 |
| Trypsin Neutralization Solution | cAP-28 |
| ITS (100x) | cAP-26 |
| L-Glutamine-MAXIMUM (100x) | cAP-27 |
| Human Plasma Fibronectin Solution | cAP-42 |
| Handling human tissue derived products is potentially bio-hazardous, despite tested negative for HIV, HBV and HCV DNA; nonetheless, proper precautions must be taken to avoid inadvertent exposure. |
