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Human Colon Cancer Organoid

Size Price Quantity
1.0 Frozen Vial $950.00
Name of ProductsHuman Colon Cancer Organoid
Catalogue NumbercAP-0205
Product FormatFrozen Vial
Cell Number> 3 x 10[6] cells/vial
Human Colon cancer tissue was procured from an approved source. Cells were isolated by collagenase digestion and cells were cultures with Matrigel (Corning) in 50/50 DMEM/F12 supplemented with 5%FBS, ROCK inhibitor (to inhibit the growth of fibroblasts), and bFGF. At this stage, cells grow as small organoids. The cells were passaged for a couple of times and cryopreserved. Organoids are cryopreserved in a commercial freezing medium with about 3 x 10[6] cancer cells as organoids per vial.
1. Colon Cancer Organoid are negative for HIV-1, HBV, HCV, and mycoplasma
Cells are offered for Research Use Only.
Frozen Vials in a Dry Ice Package.
When you receive the dry ice package with cells in frozen vials, transfer the frozen vials of cells into a -80C freezer for short period storage or a liquid nitrogen tank for long- term storage.
A) Frozen organoids are thawed at 37C with gentle shaking.
B)The contents of the vial are transferred to 5ml of tissue culture medium dropwise and spun at 500g and the viability after thawing is usually 85 to 90%.
C)The organoids are plated in Ultra Low Binding tissue culture vessels (Corning). The cells are plated in 50/50 DMEM supplemented with 5% FBS, 10 ng/ml of basis FGF and antibiotics (25 ug/ml of gentamicin and 2.5 ug/ml of Amphotericin B).
D)The organoids can either be used immediately for experiments or maintained for several weeks in this medium with periodic medium change (The organoids grow very slowly).
Quick Coating SolutioncAP-01
Astrocyte Growth MediumcAP-29
Astrocyte Basal MediumcAP-29B
HBSS w/o Ca2+, Mg2+cAP-11
Cell Freezing Solution (FBS)cAP-22
Cell Freezing Solution (Non-FBS)cAP-22B
Trypsin/EDTA SolutioncAP-23
Trypsin Neutralization SolutioncAP-28
ITS (100x)cAP-26
L-Glutamine-MAXIMUM (100x) cAP-27
Human Plasma Fibronectin SolutioncAP-42
Handling human tissue derived products is potentially bio-hazardous, despite tested negative for HIV, HBV and HCV DNA; nonetheless, proper precautions must be taken to avoid inadvertent exposure.
SPECIAL NOTES: 1. We strongly advise our customers using our own medium and related accessory products; 2. The quality of cells, in term of their growth features stated in our datasheets, will not be waranteed if NON-ANGIO-PROTEOMIE's Cell Growth Medium is used; 3.Due to the sensitive nature of primary cells and cell lines, all quality related issues about the cells products must be reported back to us within ONE month period after receiving the products, no quality warranty (i.e. replacement of cells) will be provided after the ONE-Month period.