Goat anti-PCNA (aa111-122), Biotinylated Antibody

Item#:

dAP-3487

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-PCNA (aa111-122), Biotinylated Antibody
Principle Name	PCNA (aa111-122), Biotinylated
Official Symbol	PCNA
All Name and Symbols	PCNA; proliferating cell nuclear antigen; MGC8367; DNA polymerase delta auxiliary protein; OTTHUMP00000030189; OTTHUMP00000030190; cyclin
Accession Number(s)	NP_002583.1
Peptide Sequence	EAPNQEKVSDYE., is from internal region
Human Gene ID(s)	5111
Non-Human GeneID(s)	18538 (mouse); 25737 (rat);
Important Comments	Reported variants represent identical protein
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Approx 30kDa band observed in lysates of cell line Jurkat (calculated MW of 28.8kDa according to Human NP_002583.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Gilljam et al, , J C
Reference(s)	Jones MJ, Colnaghi L, Huang TT. Dysregulation of DNA polymerase ¿ recruitment to replication forks results in genomic instability. EMBO J. 2011 Dec 13..PMID