Goat anti-CTCF Antibody |
| Principle Name | CTCF |
| Official Symbol | CTCF |
| All Name and Symbols | CCCTC-binding factor (zinc finger protein); 11 zinc finger transcriptional repressor; 11-zinc finger protein; CTCFL paralog; transcriptional repressor CTCF |
| Accession Number(s) | NP_006556.1 |
| Peptide Sequence | CHLPQNQTDGGE, is from internal region |
| Human Gene ID(s) | 10664 |
| Non-Human GeneID(s) | 13018 (mouse); 83726 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 1000. |
| Western Blot | Approx 150kDa band observed in lysates of cell line Jurkat and nuclear Jurkat (calculated MW of 82.8kDa according to NP_006556.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial |
| Reference(s) | Chen Q, Lin L, Smith S, Huang J, Berger SL, Zhou J. CTCF-dependent chromatin boundary element between the latency-associated transcript and ICP0 promoters in the herpes simplex virus type 1 genome. J Virol. 2007 May;81(10) |
