Goat anti-IDE (long isoform aa10-14)) Antibody

Item#:

dAP-3473

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-IDE (long isoform aa10-14)) Antibody
Principle Name	IDE (long isoform aa10-14))
Official Symbol	IDE
All Name and Symbols	IDE; insulin-degrading enzyme; INSULYSIN; Abeta-degrading protease; insulin protease; insulinase
Accession Number(s)	NP_004960.2; NP_001159418.1
Peptide Sequence	HPALPSTFRSVLGAR, is from N Terminus
Human Gene ID(s)	3416
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 2000.
Western Blot	Approx 58-60kDa band observed in cell lysates of HEK293 and HeLa calculated MW of 54.2kDa according to NP_001159418.1). Recommended concentration
Reference(s)	Leal MC, Surace EI, Holgado MP, Ferrari CC, Tarelli R, Pitossi F, Wisniewski T, Castaño EM, Morelli L. Notch signaling proteins HES-1 and Hey-1 bind to insulin degrading enzyme (IDE) proximal promoter and repress its transcription and activity