Goat anti-LAG3 / CD223 Antibody

Item#:

dAP-3467

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-LAG3 / CD223 Antibody
Principle Name	LAG3 / CD223
Official Symbol	LAG3
All Name and Symbols	LAG3; lymphocyte activating 3; CD223; lymphocyte-activation gene 3
Accession Number(s)	NP_002277.4
Peptide Sequence	EDVSQAQAGTYTCH, is from internal region
Human Gene ID(s)	3902
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognize the extra-cellular domain.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 2000.
Western Blot	Approx 55kDa band observed in Human Lymph Node and Tonsil lysates (calculated MW of 57.4kDa according to NP_002277.4). Recommended concentration
Reference(s)	Tian X, Zhang A, Qiu C, Wang W, Yang Y, Qiu C, Liu A, Zhu L, Yuan S, Hu H, Wang W, Wei Q, Zhang X, Xu J. The upregulation of LAG-3 on T cells defines a subpopulation with functional exhaustion and correlates with disease progression in HIV-infected subjects. Journal of immunology (Baltimore, Md.