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Goat anti-CTCF (isoform 1) Antibody
Item#:
dAP-3465
Size Price Quantity
50.0 ug $310.00
Goat anti-CTCF (isoform 1) Antibody
Principle Name CTCF (isoform 1)
Official Symbol CTCF
All Name and Symbols CTCF; CCCTC-binding factor (zinc finger protein); 11 zinc finger transcriptional repressor; 11-zinc finger protein; CTCFL paralog; transcriptional repressor CTCF
Accession Number(s) NP_006556.1
Peptide Sequence KKSKLRYTEEGKD, is from internal region
Human Gene ID(s) 10664
Non-Human GeneID(s) 13018 (mouse); 83726 (rat);
Important Comments This antibody is expected to recognize reported isoform 1 (NP_006556.1) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 4000.
Western Blot Approx 150kDa band observed in lysates of cell line Jurkat and nuclear Jurkat (calculated MW of 82.8kDa according to NP_006556.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial
Reference(s) Lee BK, Iyer VR. Genome-wide studies of CCCTC-binding factor (CTCF) and cohesin provide insight into chromatin structure and regulation. The Journal of biological chemistry 2012 Sep 287 (37)

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