Goat anti-MDH2 (aa221-232) Antibody

Item#:

dAP-3450

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-MDH2 (aa221-232) Antibody
Principle Name	MDH2 (aa221-232)
Official Symbol	MDH2
All Name and Symbols	MDH2; malate dehydrogenase 2, NAD (mitochondrial); M-MDH; MDH; MGC
Accession Number(s)	NP_005909.2
Peptide Sequence	DQLTALTGRIQE, is from internal region
Human Gene ID(s)	4191
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Approx 36-37kDa band observed in lysates of cell lines HeLa, HepG2 and K562, and approx 35kDa band observed in Human Heart and Liver lysates (calculated MW of 35.5kDa according to NP_005909.2). Recommended concentration
Reference(s)	Morgunov I, Srere PA. Interaction between citrate synthase and malate dehydrogenase. Substrate channeling of oxaloacetate. The Journal of biological chemistry 1998 Nov 273 (45)