Goat anti-Flotillin 1, Biotinylated Antibody

Item#:

dAP-3418

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Flotillin 1, Biotinylated Antibody
Principle Name	Flotillin 1, Biotinylated
Official Symbol	FLOT1
All Name and Symbols	FLOT1; flotillin 1; integral membrane component of caveolae
Accession Number(s)	NP_005794.1; NP_001305804.1
Peptide Sequence	CSISQVNHKPLRTA., is from C Terminus
Human Gene ID(s)	10211
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognize both reported isoforms (NP_005794.1; NP_001305804.1).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 2000.
Western Blot	Approx 48kDa band observed in lysates of cell line K562 (calculated MW of 47.4kDa according to NP_005794.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration
Reference(s)	Bickel PE, Scherer PE, Schnitzer JE, Oh P, Lisanti MP, Lodish HF. Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins. The Journal of biological chemistry 1997 May 272 (21)