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Goat anti-ALPL (aa42-53) Antibody
Item#:
dAP-3414
Size Price Quantity
50.0 ug $310.00
Goat anti-ALPL (aa42-53) Antibody
Principle Name ALPL (aa42-53)
Official Symbol ALPL
All Name and Symbols ALPL; alkaline phosphatase, liver/bone/kidney; AP-TNAP; APTNAP; HOPS; TNAP; TNSALP; alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; glycerophosphatase; liver/bone/kidney-type
Accession Number(s) NP_000469.3
Peptide Sequence ELQKLNTNVAKN, is from internal region (near N terminus)
Human Gene ID(s) 249
Non-Human GeneID(s) 11647 (mouse); 25586 (rat);
Important Comments This antibody is expected to recognize isoform 1 (NP_000469.3) only. The immunizing peptide represents the N terminus of the mature protein.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 64000.
Western Blot Approx 55kDa band observed in Human Kidney, Lung and Adrenal Gland lysates (calculated MW of 57.3kDa according to NP_000469.3). Recommended concentration
Reference(s) Fukunaka A, Kurokawa Y, Teranishi F, Sekler I, Oda K, Ackland ML, Faundez V, Hiromura M, Masuda S, Nagao M, Enomoto S, Kambe T. Tissue nonspecific alkaline phosphatase is activated via a two-step mechanism by zinc transport complexes in the early secretory pathway. The Journal of biological chemistry 2011 May 286 (18)

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