Goat anti-Glypican-1 Antibody

Item#:

dAP-3397

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Glypican-1 Antibody
Principle Name	Glypican-1
Official Symbol	GPC1
All Name and Symbols	GPC1; glypican 1; glypican; glypican proteoglycan 1; glypican-1
Accession Number(s)	NP_002072.2
Peptide Sequence	TDKFWGTSGVES, is from internal region
Human Gene ID(s)	2817
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Approx 60kDa band observed in lysates of cell lines HeLa and K562 (calculated MW of 61.7kDa according to NP_002072.2). Recommended concentration
Reference(s)	Melo SA, Luecke LB, Kahlert C, Fernandez AF, Gammon ST, Kaye J, LeBleu VS, Mittendorf EA, Weitz J, Rahbari N, Reissfelder C, Pilarsky C, Fraga MF, Piwnica-Worms D, Kalluri R. Glypican-1 identifies cancer exosomes and detects early pancreatic cancer. Nature. 2015 Jul 9;523(7559)