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Goat anti-SIAH1, Biotinylated Antibody
Item#:
dAP-3380
Size Price Quantity
50.0 ug $310.00
Goat anti-SIAH1, Biotinylated Antibody
Principle Name SIAH1, Biotinylated
Official Symbol SIAH1
All Name and Symbols SIAH1; siah E3 ubiquitin protein ligase 1; SIAH1A; seven in absentia homolog 1; siah-1a
Accession Number(s) NP_003022.3; NP_001006611.1
Peptide Sequence SRQTATALPTGTSKC., is from N Terminus
Human Gene ID(s) 6477
Non-Human GeneID(s) 20437 (mouse); 140941 (rat);
Important Comments This antibody is expected to recognize both reported isoforms (NP_003022.3; NP_001006611.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IP
Tested Species Human, Mouse, Rat
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Approx 28kDa band observed in Mouse Brain lysates (calculated MW of 31.1kDa according to Mouse NP_033198.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration
Reference(s) Susini L, Passer BJ, Amzallag-Elbaz N, Juven-Gershon T, Prieur S, Privat N, Tuynder M, Gendron MC, Israël A, Amson R, Oren M, Telerman A. Siah-1 binds and regulates the function of Numb. Proceedings of the National Academy of Sciences of the United States of America 2001 Dec 98 (26)

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