Goat anti-PLK1 Antibody |
| Principle Name | PLK1 |
| Official Symbol | PLK1 |
| All Name and Symbols | PLK1; polo-like kinase 1 (Drosophila) ; HGNC |
| Accession Number(s) | NP_005021.2 |
| Peptide Sequence | DKLLSSRSASNRLK, is from C Terminus |
| Human Gene ID(s) | 5347 |
| Non-Human GeneID(s) | 18817 (mouse); 25515 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 65kDa band observed in lysates of cell line K562 (calculated MW of 68.3kDa according to NP_005021.2). Recommended concentration |
| Reference(s) | Preisinger C, Korner R, Wind M, Lehmann WD, Kopajtich R, Barr FA. Plk1 docking to GRASP65 phosphorylated by Cdk1 suggests a mechanism for Golgi checkpoint signalling. EMBO J. 2005 Feb 23;24(4) |
