Goat anti-PCNA, Biotinylated Antibody

Item#:

dAP-3362

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-PCNA, Biotinylated Antibody
Principle Name	PCNA, Biotinylated
Official Symbol	PCNA
All Name and Symbols	PCNA; proliferating cell nuclear antigen; ATLD2; DNA polymerase delta auxiliary protein; cyclin
Accession Number(s)	NP_002583.1
Peptide Sequence	NGNIKLSQTSNVD., is from internal region
Human Gene ID(s)	5111
Non-Human GeneID(s)	18538 (mouse); 25737 (rat);
Important Comments	Reported variants represent identical protein
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, EIA
Tested Species	Human, Mouse, Rat, Pig
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Approx 30kDa band observed in lysates of cell line HeLa (calculated MW of 28.8kDa according to NP_002583.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration
Reference(s)	Jones MJ, Colnaghi L, Huang TT. Dysregulation of DNA polymerase ¿ recruitment to replication forks results in genomic instability. The EMBO journal 2012 Feb 31 (4)