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Goat anti-ACAT1 (aa257-269), Biotinylated Antibody


Item#:
dAP-3353
Size Price Quantity
50.0 ug $310.00
Goat anti-ACAT1 (aa257-269), Biotinylated Antibody
Principle Name ACAT1 (aa257-269), Biotinylated
Official Symbol ACAT1
All Name and Symbols ACAT1; acetyl-CoA acetyltransferase 1; ACAT; MAT; T2; THIL; acetoacetyl Coenzyme A thiolase; acetoacetyl-CoA thiolase; acetyl-Coenzyme A acetyltransferase 1; mitochondrial acetoacetyl-CoA thiolase
Accession Number(s) NP_000010.1
Peptide Sequence KRVDFSKVPKLKT., is from internal region
Human Gene ID(s) 38
Non-Human GeneID(s) 110446 (mouse); 25014 (rat);
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IF
Tested Species Human, Mouse, Rat
Peptide ELISA antibody detection limit dilution 1 to 8000.
Western Blot Approx 45kDa band observed in Mouse Liver lysates (calculated MW of 44.8kDa according to Mouse NP_659033.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration
Reference(s) Hongo S, Watanabe T, Arita S, Kanome T, Kageyama H, Shioda S, Miyazaki A. Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages. American journal of physiology. Endocrinology and metabolism 2009 Aug 297 (2)

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