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Goat anti-VHL, Biotinylated Antibody
Item#:
dAP-3349
Size Price Quantity
50.0 ug $310.00
Goat anti-VHL, Biotinylated Antibody
Principle Name VHL, Biotinylated
Official Symbol VHL
All Name and Symbols VHL; von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase; HRCA1; RCA1; VHL1; pVHL; elongin binding protein; protein G7
Accession Number(s) NP_000542.1; NP_937799.1; XP_011532380.1
Peptide Sequence RSLVKPENYRRLD., is from internal region
Human Gene ID(s) 7428
Non-Human GeneID(s) 22346 (mouse); 24874 (rat);
Important Comments This antibody is expected to recognise both reported isoforms (NP_000542.1 and NP_937799.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, EIA
Tested Species Human, Mouse, Rat
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Approx 17kDa band observed in Human Ovary lysates (calculated MW of 19.7kDa according to NP_937799.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration
Reference(s) Kong X, Lin Z, Liang D, Fath D, Sang N, Caro J. Histone deacetylase inhibitors induce VHL and ubiquitin-independent proteasomal degradation of hypoxia-inducible factor 1alpha. Molecular and cellular biology 2006 Mar 26 (6)

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