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Goat anti-FHL1 / SLIM1, Biotinylated Antibody
Item#:
dAP-3337
Size Price Quantity
50.0 ug $310.00
Goat anti-FHL1 / SLIM1, Biotinylated Antibody
Principle Name FHL1 / SLIM1, Biotinylated
Official Symbol FHL1
All Name and Symbols FHL1; four and a half LIM domains 1; FHL-1; FHL1A; FHL1B; FLH1A; KYOT; SLIM; SLIM-1; SLIM1; SLIMMER; XMPMA; LIM protein SLIMMER; four-and-a-half Lin11, Isl-1 and Mec-3 domains 1; skeletal muscle LIM-protein 1
Accession Number(s) NP_001440.2; NP_001153171.1
Peptide Sequence CNKRFVFHNEQVY., is from internal region
Human Gene ID(s) 2273
Non-Human GeneID(s) 14199 (mouse); 25177 (rat);
Important Comments This antibody is expected to recognise isoforms 2 (NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variants NP
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human, Mouse
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Approx 30kDa band observed in Human Heart lysates (calculated MW of 31.9kDa according to NP_001440.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration
Reference(s) Brown S, McGrath MJ, Ooms LM, Gurung R, Maimone MM, Mitchell CA. Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1. Localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in the cytoskeleton and in nuclear The Journal of biological chemistry 1999 Sep 274 (38)

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