Goat anti-HMGCS1 Antibody

Item#:

dAP-3323

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-HMGCS1 Antibody
Principle Name	HMGCS1
Official Symbol	HMGCS1
All Name and Symbols	HMGCS1; 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble); HMGCS; 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase; 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble); hydroxymethylglutaryl-CoA synthase, cytoplasmic
Accession Number(s)	NP_002121.4
Peptide Sequence	ERNNLSYDCIGRLE, is from internal region
Human Gene ID(s)	3157
Non-Human GeneID(s)
Important Comments	Reported variants represent identical protein
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Approx 57kDa band observed in lysates of cell lines HeLa, HepG2, and K562 (calculated MW of 57.3kDa according to NP_002121.4). Recommended concentration
Reference(s)	Shafqat N, Turnbull A, Zschocke J, Oppermann U, Yue WW. Crystal structures of human HMG-CoA synthase isoforms provide insights into inherited ketogenesis disorders and inhibitor design. Journal of molecular biology 2010 May 398 (4)