Goat anti-MIF, Biotinylated Antibody

Item#:

dAP-3308

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-MIF, Biotinylated Antibody
Principle Name	MIF, Biotinylated
Official Symbol	MIF
All Name and Symbols	MIF; macrophage migration inhibitory factor (glycosylation-inhibiting factor); GIF; GLIF; MMIF; L-dopachrome isomerase; L-dopachrome tautomerase; phenylpyruvate tautomerase
Accession Number(s)	NP_002406.1
Peptide Sequence	NAANVGWNNSTFA., is from C Terminus
Human Gene ID(s)	4282
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, EIA
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Approx 13kDa band observed in lysates of cell line Daudi (calculated MW of 12.5kDa according to NP_002406.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration
Reference(s)	Church TS, Willis MS, Priest EL, Lamonte MJ, Earnest CP, Wilkinson WJ, Wilson DA, Giroir BP. Obesity, macrophage migration inhibitory factor, and weight loss. International journal of obesity (2005) 2005 Jun 29 (6)