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Goat anti-GAPDH (C Terminus) Loading Control, biotinylated Antibody
Item#:
dAP-3294
Size Price Quantity
50.0 ug $310.00
Goat anti-GAPDH (C Terminus) Loading Control, biotinylated Antibody
Principle Name GAPDH (C Terminus) Loading Control, biotinylated
Official Symbol GAPDH
All Name and Symbols GAPDH; glyceraldehyde-3-phosphate dehydrogenase; G3PD; GAPD; HEL-S-162eP; aging-associated gene 9 protein; epididymis secretory sperm binding protein Li 162eP; peptidyl-cysteine S-nitrosylase GAPDH
Accession Number(s) NP_002037.2
Peptide Sequence HQVVSSDFNSDT., is from C Terminus
Human Gene ID(s) 2597
Non-Human GeneID(s)
Important Comments GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human, Mouse, Rat
Peptide ELISA antibody detection limit dilution 1 to 64000.
Western Blot Approx 35kDa band observed in lysates of cel line HEK293 (calculated MW of 36.1kDa according to NP_002037.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration
Reference(s) Burke JR, Enghild JJ, Martin ME, Jou YS, Myers RM, Roses AD, Vance JM, Strittmatter WJ. Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH. Nature medicine 1996 Mar 2 (3)

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