Goat anti-HLA-B Antibody |
| Principle Name | HLA-B |
| Official Symbol | HLA-B |
| All Name and Symbols | HLA-B; major histocompatibility complex, class I, B; AS; HLAB; SPDA1; HLA class I histocompatibility antigen, B alpha chain; MHC Class I HLA heavy chain; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC class I antigen GN0010 |
| Accession Number(s) | NP_005505.2 |
| Peptide Sequence | DRNTQIYKAQAQT , is from internal region |
| Human Gene ID(s) | 3106 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Approx 40+45kDa bands observed in lysates of cell line Jurkat (calculated MW of 40.5kDa according to NP_005505.2). The observed 45kDa band represents the glycosylated form. Recommended concentration |
| Reference(s) | Owen MJ, Kissonerghis AM, Lodish HF. Biosynthesis of HLA-A and HLA-B antigens in vivo. The Journal of biological chemistry 1980 Oct 255 (20) |