Goat anti-EGFR Antibody |
| Principle Name | EGFR |
| Official Symbol | EGFR |
| All Name and Symbols | EGFR; epidermal growth factor receptor; erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian ; HGNC |
| Accession Number(s) | NP_005219.2 |
| Peptide Sequence | QKGSHQISLDNPD, is from internal region |
| Human Gene ID(s) | 1956 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise isoform a (NP_005219.2) only. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 170kDa band observed in lysates of cell line HeLa (calculated MW of 134kDa according to NP_005219.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration |
| Reference(s) | Shigematsu H, Lin L, Takahashi T, Nomura M, Suzuki M, Wistuba II, Fong KM, Lee H, Toyooka S, Shimizu N, Fujisawa T, Feng Z, Roth JA, Herz J, Minna JD, Gazdar AF. Clinical and biological features associated with epidermal growth factor receptor gene mutations in lung cancers. J Natl Cancer Inst. 2005 Mar 2;97(5) |
