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Goat anti-dysferlin Antibody
Item#:
dAP-3271
Size Price Quantity
50.0 ug $310.00
Goat anti-dysferlin Antibody
Principle Name dysferlin
Official Symbol DYSF
All Name and Symbols DYSF; dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive); FER1L1; LGMD2B; MMD1; dysferlin; dystrophy-associated fer-1-like 1; dystrophy-associated fer-1-like protein; fer-1-like protein 1
Accession Number(s) NP_001124459.1; NP_001123927.1; NP_001124458.1; NP_001124457.1; NP_001124456.1; NP_001124455.1; NP_001124454.1; NP_003485.1; NP_001124448.1; NP_001124449.1; NP_001124450.1; NP_001124451.1; NP_001124452.1; NP_001124453.1
Peptide Sequence HLFCQQHRVKAP, is from internal region
Human Gene ID(s) 8291
Non-Human GeneID(s) 312492 (rat);
Important Comments This antibody is expected to recognize all 14 reported isoforms.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA
Tested Species
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Preliminary experiments in Human and rodent Heart and Skeletal Muscle lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results
Reference(s) de Morrée A, Flix B, Bagaric I, Wang J, van den Boogaard M, Grand Moursel L, Frants RR, Illa I, Gallardo E, Toes R, van der Maarel SM. Dysferlin regulates cell adhesion in human monocytes. The Journal of biological chemistry 2013 May 288 (20)

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