Goat anti-GNL3 Antibody |
| Principle Name | GNL3 |
| Official Symbol | GNL3 |
| All Name and Symbols | GNL3; guanine nucleotide binding protein-like 3 (nucleolar); C77032; E2IG3; NNP47; NS; E2-induced gene 3 protein; estradiol-induced nucleotide binding protein; guanine nucleotide-binding protein-like 3; novel nucleolar protein 47; nucleolar GTP-binding pr |
| Accession Number(s) | NP_055181.3; NP_996561.1 |
| Peptide Sequence | EKNNAQSIRAIKGPH, is from internal region |
| Human Gene ID(s) | 26354 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_055181.3; NP_996561.1). Reported variants represent identical protein |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 65+75kDa bands observed in nuclear lysates of cell lines HeLa, HepG2 and K562 (calculated MW of 62.0kDa according to NP_055181.3). The observed 75kDa band likely represents the acetylated form. Recommended concentration |
| Reference(s) | Yoshida R, Fujimoto T, Kudoh S, Nagata M, Nakayama H, Shinohara M, Ito T. Nucleostemin affects the proliferation but not differentiation of oral squamous cell carcinoma cells. Cancer science 2011 Jul 102 (7) |
