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Goat anti-Centromere protein E (aa2593-2604) Antibody
Item#:
dAP-3237
Size Price Quantity
50.0 ug $310.00
Goat anti-Centromere protein E (aa2593-2604) Antibody
Principle Name Centromere protein E (aa2593-2604)
Official Symbol CENPE
All Name and Symbols CENPE; centromere protein E, 312kDa; CENP-E; KIF10; PPP1R61; Centromere autoantigen E (312kD); centromere-associated protein E; kinesin family member 10; kinesin-related protein CENPE; protein phosphatase 1, regulatory subunit 61
Accession Number(s) NP_001804.2; NP_001273663.1
Peptide Sequence KREAHKQVTCEN, is from internal region
Human Gene ID(s) 1062
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize both reported isoforms (NP_001804.2; NP_001273663.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA
Tested Species
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 316kDa according to NP_001804.2. Therefore we cannot recommend an optimal concentration and the antibody i
Reference(s) Sardar HS, Gilbert SP. Microtubule capture by mitotic kinesin centromere protein E (CENP-E). The Journal of biological chemistry 2012 Jul 287 (30)

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