Goat anti-ATG4A Antibody |
| Principle Name | ATG4A |
| Official Symbol | ATG4A |
| All Name and Symbols | ATG4A; ATG4 autophagy related 4 homolog A (S. cerevisiae); APG4A; AUTL2; APG4 autophagy 4 homolog A; AUT-like 2, cysteine endopeptidase; OTTHUMP00000062893; autophagin 2; autophagy-related cysteine endopeptidase 2 |
| Accession Number(s) | NP_443168.2; NP_840054.1 |
| Peptide Sequence | TEENGTVNDQTFHC, is from internal region |
| Human Gene ID(s) | 115201 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_443168.2; NP_840054.1) |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 1000. |
| Immunohistochemistry | In paraffin embedded Human Liver shows textured cytoplasm staining in the hepatocytes. Recommended concentration, 2-4µg/ml. |
| Reference(s) | Scherz-Shouval R, Sagiv Y, Shorer H, Elazar Z The COOH terminus of GATE-16, an intra-Golgi transport modulator, is cleaved by the human cysteine protease HsApg4A J Biol Chem. 2003 Apr 18;278(16) |
