Goat anti-AIFM1 Antibody |
| Principle Name | AIFM1 |
| Official Symbol | AIFM1 |
| All Name and Symbols | AIFM1; apoptosis-inducing factor, mitochondrion-associated, 1; AIF; CMTX4; COWCK; COXPD6; PDCD8; apoptosis-inducing factor 1, mitochondrial; programmed cell death 8 (apoptosis-inducing factor); striatal apoptosis-inducing factor |
| Accession Number(s) | NP_004199.1; NP_665811.1; NP_665812.1; NP_001124318.1 |
| Peptide Sequence | NEVAKLFNIHED, is from C Terminus |
| Human Gene ID(s) | 9131 |
| Non-Human GeneID(s) | 26926 (mouse); 83533 (rat); |
| Important Comments | This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1), isoform 3 (NP_665812.1) and isoform 4 (NP_001124318.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IF, WB |
| Tested Species | Human, Mouse, Rat, Pig |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 70kDa band observed in lysates of cell line Jurkat and NIH3T3, and in Mouse and Rat Heart and Kidney lysates. Approx. 65kDa observed in Pig Heart lysates (calculated MW of 66.9kDa according to Human NP_004199.1 and 66.8kDa according |
| Reference(s) | Lewis EM, Wilkinson AS, Jackson JS, Mehra R, Varambally S, Chinnaiyan AM, Wilkinson JC. The enzymatic activity of apoptosis-inducing factor supports energy metabolism benefiting the growth and invasiveness of advanced prostate cancer cells. The Journal of biological chemistry 2012 Dec 287 (52) |
