Goat anti-Vimentin Antibody

Item#:

dAP-3207

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Vimentin Antibody
Principle Name	Vimentin
Official Symbol	VIM
All Name and Symbols	VIM; vimentin; FLJ36605
Accession Number(s)	NP_003371.2
Peptide Sequence	QVINETSQHHDDLE, is from C Terminus
Human Gene ID(s)	7431
Non-Human GeneID(s)	22352 (mouse); 81818 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, FC
Tested Species	Human, Mouse, Rat
Peptide ELISA	antibody detection limit dilution 1 to 4000.
Western Blot	Approx 55kDa band observed in lysates of cell line HeLa and Jurkat and in Mouse Ovary lysates, and approx. 55-60kDa band in Rat Ovary lysates (calculated MW of 53.7kDa according to Human NP_003371.2, Mouse NP_035831.2 and Rat NP_112402.1).
Reference(s)	Maraina CH, Nurdayana AK, Rusni D, Azwany Y. Diagnostic value of anti-modified citrullinated vimentin in rheumatoid arthritis. Int J Rheum Dis. 2010 Oct;13(4)