Goat anti-CD3EAP Antibody

Item#:

dAP-3197

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-CD3EAP Antibody
Principle Name	CD3EAP
Official Symbol	CD3EAP
All Name and Symbols	CD3EAP; CD3e molecule, epsilon associated protein; ASE-1; ASE1; CAST; PAF49; A34.5; CD3 epsilon associated protein; CD3-epsilon-associated protein; CD3E-associated protein; CD3e antigen, epsilon polypeptide associated protein; DNA-directed RNA polymerase
Accession Number(s)	NP_036231.1
Peptide Sequence	PEDKTVKQEQINTEP, is from internal region
Human Gene ID(s)	10849
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 4000.
Western Blot	Preliminary experiments in nuclear lysates of cell lines HeLa, Jurkat and A431 gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results
Reference(s)	Yin J, Guo L, Wang C, Wang H, Ma Y, Liu J, Liang D, Ma J, Zhao Y. Effects of PPP1R13L and CD3EAP variants on lung cancer susceptibility among nonsmoking Chinese women. Gene 2013 Jul 524 (2)