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Goat anti-CD3E (cytoplasmic) Antibody


Item#:
dAP-3189
Size Price Quantity
50.0 ug $310.00
Goat anti-CD3E (cytoplasmic) Antibody
Principle Name CD3E (cytoplasmic)
Official Symbol CD3E
All Name and Symbols CD3E; CD3e molecule, epsilon (CD3-TCR complex); IMD18; T3E; TCRE; CD3-epsilon; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycopro
Accession Number(s) NP_000724.1
Peptide Sequence RKGQRDLYSGLNQR, is from internal region
Human Gene ID(s) 916
Non-Human GeneID(s) 315609 (rat);
Important Comments This antibody is designed to recognize the cytoplasmic domain (aa153-207) of the protein.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Approx 22kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 23.1kDa according to NP_000724.1). Recommended concentration
Reference(s) Gagnon E, Schubert DA, Gordo S, Chu HH, Wucherpfennig KW. Local changes in lipid environment of TCR microclusters regulate membrane binding by the CD3e cytoplasmic domain. The Journal of experimental medicine 2012 Dec 209 (13)

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