Goat anti-NME1 Antibody |
| Principle Name | NME1 |
| Official Symbol | NME1 |
| All Name and Symbols | NME1; AWD; GAAD; NB; NBS; NDPK-A; NDPKA; NM23; NM23-H1; non-metastatic cells 1, protein (NM23A) expressed in; NDP kinase A; OTTHUMP00000174772 |
| Accession Number(s) | NP_937818.1; NP_000260.1 |
| Peptide Sequence | DYTSCAQNWIYE, is from C Terminus |
| Human Gene ID(s) | 4830 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_937818.1; NP_000260.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 20+18kDa bands observed in lysates of cell lines A549, HeLa and HepG2 (calculated MW of 19.7kDa according to NP_937818.1 and 17.1kDa according to NP_000260.1). Recommended concentration |
| Reference(s) | Su Kim D, Choi YD, Moon M, Kang S, Lim JB, Kim KM, Park KM, Cho NH.Su Kim D, Choi YD, Moon M, Kang S, Lim JB, Kim KM, Park KM, Cho NH.Su Kim D, Choi YD, Moon M, Kang S, Lim JB, Kim KM, Park KM, Cho NH. Composite three-marker assay for early detection of kidney cancer. Cancer epidemiology, biomarkers & prevention |
