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Goat anti-Nanog (mouse aa270-284) Antibody
Item#:
dAP-3185
Size Price Quantity
50.0 ug $310.00
Goat anti-Nanog (mouse aa270-284) Antibody
Principle Name Nanog (mouse aa270-284)
Official Symbol Nanog
All Name and Symbols Nanog; Nanog homeobox; 2410002E02Rik, ENK, ecat4; ES cell-associated protein 4; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein; homeobox protein NANOG; homeobox transcription factor Nanog
Accession Number(s) NP_082292.1
Peptide Sequence DLEVNLEATRESHAH, is from internal region
Human Gene ID(s)
Non-Human GeneID(s) 71950 (mouse);
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Mouse
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Approx 40kDa band observed in lysates of cell line NIH3T3 (calculated MW of 34.6kDa according to NP_082292.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zbinden et al, EMBO J. 201
Reference(s) Han J, Mistriotis P, Lei P, Wang D, Liu S, Andreadis ST. Nanog reverses the effects of organismal aging on mesenchymal stem cell proliferation and myogenic differentiation potential. Stem cells (Dayton, Ohio) 2012 Dec 30 (12)

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