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Goat anti-PHF19 (isoform b) Antibody
Item#:
dAP-3166
Size Price Quantity
50.0 ug $310.00
Goat anti-PHF19 (isoform b) Antibody
Principle Name PHF19 (isoform b)
Official Symbol PHF19
All Name and Symbols PHF19; PHD finger protein 19; MTF2L1; PCL3; TDRD19B; hPCL3; polycomb like 3; polycomb-like 3; polycomb-like protein 3; tudor domain containing 19B
Accession Number(s) NP_001009936.1
Peptide Sequence DQRLPSQSLSSKQKGH, is from internal region (near C Terminus)
Human Gene ID(s) 26147
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize both reported isoforms (NP_056466.1; NP_001009936.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA
Tested Species
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Preliminary experiments in Human Kidney, Skeletal Muscle and Placenta lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results
Reference(s) Brien GL, Gambero G, O'Connell DJ, Jerman E, Turner SA, Egan CM, Dunne EJ, Jurgens MC, Wynne K, Piao L, Lohan AJ, Ferguson N, Shi X, Sinha KM, Loftus BJ, Cagney G, Bracken AP. Polycomb PHF19 binds H3K36me3 and recruits PRC2 and demethylase NO66 to embryonic stem cell genes during differentiation. Nature structural & molecular biology 2012 Dec 19 (12)

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