Goat anti-EEA1 (aa821-835) Antibody

Item#:

dAP-3157

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-EEA1 (aa821-835) Antibody
Principle Name	EEA1 (aa821-835)
Official Symbol	EEA1
All Name and Symbols	EEA1; early endosome antigen 1; MST105; MSTP105; ZFYVE2; early endosome antigen 1, 162kD; early endosome-associated protein; endosome-associated protein p162; zinc finger FYVE domain-containing protein 2
Accession Number(s)	NP_003557.2
Peptide Sequence	QETKIQHEELNNRIQ, is from internal region
Human Gene ID(s)	8411
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Approx 200kDa band observed in lysates of cell line HEK293 (calculated MW of 162kDa according to NP_003557.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommende
Reference(s)	Mishra A, Eathiraj S, Corvera S, Lambright DG. Structural basis for Rab GTPase recognition and endosome tethering by the C2H2 zinc finger of Early Endosomal Autoantigen 1 (EEA1). Proc Natl Acad Sci U S A. 2010 Jun 15;107(24)