Goat anti-CD47 Antibody |
| Principle Name | CD47 |
| Official Symbol | CD47 |
| All Name and Symbols | CD47; CD47 molecule; IAP; MER6; OA3; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 glycoprotein; Rh-related antigen; antigen identified by monoclonal antibody 1D8; antigenic surface determinant protein OA3; integrin associ |
| Accession Number(s) | NP_001768.1; NP_942088.1 |
| Peptide Sequence | SNQKTIQPPRK, is from internal region (near C terminus) |
| Human Gene ID(s) | 961 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_001768.1; NP_942088.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 50kDa band observed in Human Hippocampus lysates and 60kDa in lysates of cell lines HeLa and Jurkat (calculated MW of 35.2kDa according to NP_001768.1). The observed molecular weights correspond to glycosylated forms. Recommended con |
| Reference(s) | Azcutia V, Stefanidakis M, Tsuboi N, Mayadas T, Croce KJ, Fukuda D, Aikawa M, Newton G, Luscinskas FW. Endothelial CD47 promotes vascular endothelial-cadherin tyrosine phosphorylation and participates in T cell recruitment at sites of inflammation in vivo. Journal of immunology (Baltimore, Md. |
