Goat anti-BMI1 Antibody |
| Principle Name | BMI1 |
| Official Symbol | BMI1 |
| All Name and Symbols | BMI1; BMI1 polycomb ring finger oncogene; FLVI2/BMI1; PCGF4; RNF51; B lymphoma Mo-MLV insertion region 1 homolog; flvi-2/bmi-1; murine leukemia viral (bmi-1) oncogene homolog; polycomb complex protein BMI-1; polycomb group RING finger protein 4; polycomb |
| Accession Number(s) | NP_005171.4 |
| Peptide Sequence | NHQSSFANRPRKS, is from C Terminus |
| Human Gene ID(s) | 648 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 40kDa band observed in nuclear lysates of cell line K562 (calculated MW of 36.9kDa according to NP_005171.4). This band corresponds to earlier findings in literature with different antibodies (Chagraoui et al, Genes Dev. 2006 Aug 1;2 |
| Reference(s) | Cho JH, Dimri M, Dimri GP. A positive feedback loop regulates the expression of polycomb group protein BMI1 via WNT signaling pathway. The Journal of biological chemistry 2013 Feb 288 (5) |
